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Analysis of PRELP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0590-LGZ)

Introduction

Official Full Name: proline and arginine rich end leucine rich repeat protein<br />Also known as: MST161; SLRR2A; MSTP161<br />The protein encoded by this gene is a leucine-rich repeat protein present in the extracellular matrix of connective tissue. The protein functions as a molecule that anchors the basement membrane to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membrane and type II collagen to cartilage. It also binds basement membrane heparan sulfate proteoglycan. This protein is thought to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which has been reported to lack collagen binding in basement membrane and cartilage. Alternative splicing transcript variants encoding the same protein have been observed.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements