Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of EGF (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0136-LGZ)

Introduction

Human Epidermal growth factor (EGF) and TGFα, Vaccinia growth factor, amphiregulin belong to the same family and can bind to the same 170KDa receptor. EGF is a 130KDa precursor when it is first synthesized, and the mature protein has only 54 amino acids and a molecular weight of 6KDa. EGF can promote the growth and differentiation of various cells and tissues, such as epithelial differentiation, vascular regeneration, mesenchymal growth, etc.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Immunology/Inflammation, Toxicology, Cytokines & Chemokines, Kidney Injury

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Urine
Advertisement