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Analysis of Endoglin (Human) by ELISA (CAT#: STEM-MB-0780-LGZ)

Introduction

Endoglin, also known as CD105, is a type I transmembrane glycoprotein located on the surface of the cell membrane and is one of the TGF-β receptor complexes. It is involved in the regulation of responses binding to TGF-β1, TGF-β3, Activin-A, BMP-2 and BMP-7. Endoglin is speculated to be involved in the formation of the cytoskeleton affecting cell morphology and migration. It plays a role in cardiovascular system development and vascular remodeling.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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