Analysis of EPOR/erythropoietin receptor by ELISA (CAT#: STEM-MB-1584-LGZ)

Introduction

The erythropoietin receptor (EPOR) is encoded by the EPOR gene in humans. It is a polypeptide with a molecular weight of 59 kDa and is a member of the cytokine receptor family. The cytoplasmic domain of EPOR contains many Janus kinase 2 phosphorylated tyrosines that serve as anchor sites for intracellular pathway signaling and activators of transcription. Deletion of the erythropoietin receptor causes erythroleukemia and familial polycythemia. Excess production of red blood cells can lead to adverse cardiovascular events such as blood clots and strokes. EPOR signaling prevents neuronal death and ischemic injury.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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