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Analysis of Folate R alpha (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0188-LGZ)

Introduction

Folate is an essential component of cellular metabolism and DNA synthesis and repair. It participates in the necessary one-carbon transfer reaction and is a vitamin required by both normal and tumor cells. Folate enters cells through two distinct systems: the reduced folate carrier and the folate receptor system utilizing a bidirectional anion exchange mechanism. Folate Receptor alpha (Folate R alpha) is a membrane-bound member of the folate receptor family that facilitates folate transport through a mechanism called phagocytosis, in which the receptor is internalized and then recycled back to the cell membrane.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Neurobiology/Neurodegeneration

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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