Analysis of HE4/WFDC2 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0247-LGZ)

Introduction

HE4 was originally isolated from human epididymal epithelial cells. It is a secreted glycoprotein encoded by the WFDC2 gene, with a relative molecular weight of 25 kDa, and is located on chromosome 20q12-13.1. As a new biomarker, HE4 has been used in the diagnosis of ovarian cancer.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Toxicology

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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