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Analysis of IgA (Human, Non-human primate) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0274-LGZ)

Introduction

Immunoglobulin A (IgA) is divided into serotype and secretory type. It is the antibody second only to IgG in serum, accounting for 15%-25% of the total immunoglobulin. It has a high content in extraneous secretions, such as colostrum, saliva, tears, intestinal secretions and bronchial secretions, and plays an important role in preventing infection. Secretory IgA is a dimer with stable properties and high local concentrations. It is the main immunoglobulin in mucous secretions, including tears, saliva, sweat, colostrum and secretions of urogenital tract, gastrointestinal tract, prostate and respiratory epithelium.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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