Analysis of TNF-RI (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0591-LGZ)
Introduction
TNF RII is also known as TNFRSF1B. This protein forms a heterocomplex with TNF RI, mediating the recruitment of two anti-apoptotic proteins c-IAP1 and c-IAP2 with E3 ubiquitin ligase activity.
MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.
Applications
Immunology/Inflammation
Procedure
1. Coat the capture antibody on the well plate. 2. Add samples/calibrators. 3. Add detection antibody. 4. Read the plate and analyze the data.