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Analysis of GDF-15 (Human) by ELISA (CAT#: STEM-MB-0841-LGZ)

Introduction

GDF15 is a biomarker for cancer diagnosis, disease progression and prognosis. Its levels are elevated in multiple solid tumor cancer types, and it is a diagnostic biomarker for pancreatic, bowel, ovarian, prostate, primary liver, and lung cancer. It was associated with disease progression, prognosis, and overall survival of a variety of cancers, including colorectal cancer, gastric cancer, hepatocellular carcinoma, non-small cell lung cancer, urothelial/renal cell carcinoma, ovarian cancer, and breast cancer, but not with hematologic tumors.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Intracellular Signaling

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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