Analysis of Gelsolin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0205-LGZ)

Introduction

Gelsolin is a member of the gelsolin superfamily. It is an important actin-binding protein that controls the structure of actin by cutting, capping actin filaments, or nucleating actin. In addition to its role in reorganizing actin filaments, gelsolin also plays an important role in cellular activities such as cell motility and control of programmed cell death. In addition, the expression of gelsolin in tumor cells also changed. Variations in gelsolin also underlie certain genetic diseases.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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