Analysis of GFAP (Human) by ELISA (CAT#: STEM-MB-0845-LGZ)

Introduction

Glial Fibrillary Acidic Protein (GFAP) is a marker of astrocyte activation. GFAP is a type III intermediate filament protein that exists as a monomer. GFAP is mainly distributed in the astrocytes of the central nervous system, participates in the formation of the cytoskeleton and maintains its tension strength. It is also expressed in chondrocytes, fibroblasts, myoepithelial cells, lymphocytes, and hepatic stellate cells. However, the GFAP expressed in the peripheral system cannot be detected by the GFAP monoclonal antibody in the central nervous system, suggesting that there may be structural differences between the two sources of GFAP. GFAP may be specific to the nervous system.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Neuroinflammation, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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