Analysis of Glycogen by Fluorescence Microplate Reader (CAT#: STEM-MB-1509-LGZ)

Introduction

Sensitivity: 0.06 μg/mL
Detection range: 0.06-4.0 μg/mL
Precision: inter-lot difference of 6.6%, intra-lot difference of 3.4%
Detection equipment: Fluorescence Microplate Reader
Excitation wavelength: 535 nm
Emission wavelength: 587 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Glycogen produces glucose under the action of amylosidase, which is catalyzed by glucose oxidase to produce hydrogen peroxide. Under the action of peroxidase, hydrogen peroxide oxidizes the non-fluorescent probe into a fluorescent substance, and its fluorescence intensity is related to the content of glycogen.

Applications

It is suitable for the detection of glycogen content in liver and muscle tissue of animal.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: liver and muscle tissue of animal

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