Analysis of Vitamin C (VC) by Enzyme-labeled Instrument (CAT#: STEM-MB-1403-LGZ)

Introduction

Sensitivity: 0.31 μg/mL
Detection range: 0.31-17.5 μg/mL
Precision: inter-lot difference of 6.1%, intra-lot difference of 2.2%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 530-540 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

In the presence of phenanthroline, VC can reduce ferric ion to ferric ion, which can form pink complex with phenanthroline under certain circumstances, with maximum absorption wavelength at 536 nm. The content of VC can be calculated by comparing color.

Applications

Suitable for the detection of animal serum (plasma), animal and plant tissue vitamin C(VC) content.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: animal serum (plasma), animal and plant tissue

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