Analysis of Lipase (LPS) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1458-LGZ)

Introduction

Sensitivity: 0.03 U/L
Detection range: 0.03-9.41 U/L
Precision: inter-lot difference of 6%, intra-lot difference of 3%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 400-420 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Lipase can catalyze the substrate to produce sulfhydryl compounds, and the sulfhydryl compounds react with the color developing substrate DTNB to produce TNB. TNB has the maximum absorption at 412nm. By detecting the change of absorbance within a unit time, the lipase activity in the sample can be calculated.

Applications

It is used to detect lipase activity in serum, plasma, animal tissue and cell samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma, animal tissue and cell samples

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