Analysis of Granzyme B (Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0232-LGZ)

Introduction

Granzymes are exogenous serine proteases derived from cytoplasmic granules released by cytotoxic lymphocytes (CTLs) and natural killer cells (NK). It is considered that the determination of granzyme concentration in different clinical situations can be used as a new hint of health and disease.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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