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Analysis of Vitamin D Binding Protein (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0631-LGZ)

Introduction

Vitamin D binding protein (DBP) is mainly synthesized by the liver and widely distributed in many tissues and organs throughout the body. Recent studies have found that DBP not only binds, dissolves and transports vitamin D and its metabolites in the blood circulation, but also has immune regulatory functions independent of vitamin D transport, and has a certain relationship with the occurrence and development of inflammation and tumor.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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