Analysis of GRHL3 Gene (Mutation) in Inborn Gene (Mutation)tic Diseases by RT-qPCR (CAT#: STEM-MT-0090-LGZ)

Introduction

Also known as: SOM; VWS2; TFCP2L4<br />This gene encodes a member of the grainyhead family of transcription factors. The encoded protein may function as a transcription factor during development, and has been shown to stimulate migration of endothelial cells. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Inborn genetic diseases

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of ZNF496 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0265-LGZ)
Analysis of Fusarium Graminearum by Real-Time PCR Method (CAT#: STEM-MB-3431-LGZ)
Analysis of Streptococcus Iniae by Real-Time PCR Method (CAT#: STEM-MB-3121-LGZ)
Analysis of Norwalk Viruses G3 (NV-3) by Real-Time PCR Method (CAT#: STEM-MB-3235-LGZ)
Analysis of LINC02898 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1734-LGZ)
Analysis of PBXIP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0562-LGZ)
Analysis of Hyaloma Spp. by Real-Time PCR Method (CAT#: STEM-MB-3037-LGZ)
Analysis of Japanese Encephalitis Virus by Real-Time PCR Method (CAT#: STEM-MB-2886-LGZ)