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In-situ means “in the original place”. In the biology context, this means in the cell, body or tissue. In-situ PCR is a technique where PCR is carried out in a section of tissue within a cell. The tissue sections of interest are attached on a microscopic slide and incubated with all necessary reagents for PCR.
DNA present inside the cells provide the template for amplification, thus this technique is useful for finding the location of a certain gene and give information on which cells in which tissue it is present