Analysis of H3-4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1018-LGZ)

Introduction

Official Full Name: H3.4 histone, cluster member<br />Also known as: H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3<br />Histones are fundamental nuclear proteins responsible for the structure of eukaryotic chromosome fibers in the nucleosome. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). By linking DNA interactions between histone H1 and nucleosomes, chromatin fibers are further compacted to form higher-order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts of this gene lack poly-A tails; instead, they contain a palindromic termination element. This gene is separate from other H3 genes in the histone gene cluster on chromosome 6p22-p21.3.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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