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Analysis of HAVCR1/KIM-1 (Human) by ELISA (CAT#: STEM-MB-0876-LGZ)

Introduction

T cell immunoglobulin domain mucin domain protein-1 (TIM-1), also known as kidney injury factor-1 (KIM-1) and hepatitis A virus cell receptor 1 (HAVcr1), is a member of the TIM gene family, It plays a key role in regulating the activity of immune cells, especially the host immune response to virus infection. TIM-1 is preferentially expressed in Th2 cells and is a stimulatory molecule for T cell activation. TIM-1 is also involved in allergic reactions, asthma and transplant tolerance. In humans, TIM-1 serves as a receptor for cellular entry of several viruses, including hepatitis A virus, dengue virus, and Ebola virus.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Kidney Injury, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
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