Analysis of Heme Oxygenase 1 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0248-LGZ)

Introduction

Heme oxygenase (HO) is the only rate-limiting enzyme in the body that catalyzes the catabolism of heme. It oxidatively degrades heme, breaking it down into carbon monoxide, free iron, and biliverdin. HO has three isozymes, HO-1, HO-2, and HO-3, which are the products of different genes.

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Principle Applications Procedure Materials

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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