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Analysis of IFN-α2a (Non-human primate) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0267-LGZ)

Introduction

IFN is divided into three types: α, β, and γ according to its source and structure. IFN-α and IFN-β are secreted by many cell types, including lymphocytes (NK cells, B cells and T cells), macrophages, fibroblasts, bladder cells, osteoblasts, etc. They stimulate macrophages and NK cells to elicit antiviral responses, involve the IRF3/IRF7 antiviral pathway, and also have antitumor activity. IFN-α2a is a subtype of INF-α.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Rabbit Polyclonal
Recombinant Standards: Expressed in E. coli

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Cell Culture Supernatant
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