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Analysis of Neprilysin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0454-LGZ)

Introduction

Neprilysin (CD10) is a zinc metalloprotease expressed on the surface of various cells. CD10 is also identified as common acute lymphoblastic leukemia antigen (CALLA) and is expressed on the surface of lymphocytes in certain disease states. CD10 is also a major degrading enzyme of brain amyloid β-peptide.

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Principle Applications Procedure Materials

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Alzheimer’s Disease, Neurobiology/Neurodegeneration

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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