Analysis of IgM (Mouse) by ELISA (CAT#: STEM-MB-0926-LGZ)

Introduction

Immunoglobulin M (IgM) is the immunoglobulin with the largest molecular weight, which is mainly secreted and synthesized by plasma cells in the spleen and lymph nodes, and is divided into two subtypes, IgM1 and IgM2. It is mainly distributed in the serum and exists in the form of pentamer, accounting for 5% to 10% of the total serum Ig. IgM has powerful bactericidal, complement activating, immunomodulatory and agglutination effects, and is also involved in the pathological process of some autoimmune diseases and hypersensitivity reactions.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Assay Development

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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