Analysis of Ikaros (Human) by ELISA (CAT#: STEM-MB-0927-LGZ)

Introduction

Ikaros is one of the giant transcription factors that regulate the developmental process of the lymphatic system. During the development of the hematopoietic system, the Ikaros family not only exhibits its regulatory function by regulating the expression of target genes, but also interacts with the heterochromatin remodeling complex containing inert genes to regulate its transcriptional activity. And through the evolutionary analysis of Ikaros family members of different species, it is found that they are highly conserved and may be related to the origin of the adaptive immune system.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Intracellular Signaling

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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