Analysis of IL-13 (Rat) by ELISA (CAT#: STEM-MB-0937-LGZ)

Introduction

Interleukin-13 (Interleukin-13) is a cytokine of the chemokine family. IL-13 is produced by TH2 cells with a molecular weight of about 10KD. IL-13 can induce monocyte differentiation and enhance the expression of MHC class Ⅱ molecules; Inhibit the secretion of monokine induced by lps, control the inflammatory response; induce B cell proliferation and synthesis of IgE antibodies, enhance the expression of MHC Ⅱ molecules, CD23 and CD72 on the surface of B cells; cooperate with IL-2 to stimulate NK cells to produce IFN, thereby promoting monocyte-macrophage activation and TH1-type cellular immune response. It was also found recently that IL-13 also has multiple functions such as inhibiting HIV-1 replication in macrophages, inducing IL-1RA gene expression and protein synthesis in neutrophils.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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