Analysis of IL-15 (Human) by ELISA (CAT#: STEM-MB-0940-LGZ)

Introduction

Interleukin-15 is a cytokine of the chemokine family. IL-15 is a newly discovered factor that can be produced by various cells such as activated monocyte-macrophages, epidermal cells and fibroblasts. IL-15 can induce the proliferation and differentiation of B cells, and is the only cytokine that can partially replace the initial antibody production induced by IL-2. IL-15 can stimulate the proliferation of T cells and NK cells and induce the activity of LAK cells. IL-15 can also cooperate with IL-12 to stimulate NK cells to produce IFN-γ.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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