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Analysis of IL-24 by ELISA (CAT#: STEM-MB-1525-LGZ)

Introduction

Interleukin 24(IL-24), a cytokine that belongs to the IL-10 cytokine family, uses two heterodimer receptors called IL-20R1/IL-20R2 and IL-22R1/IL-20R2 for signaling. IL-24 may control cell survival and proliferation by inducing rapid activation of specific transcription factors Stat-1 and Stat-3. Mouse IL24 expresses a protein of about 35kD and is converted to a protein of 23kD by deglycation. IL24 proteins share 24-33% homology with other members of the IL10 cytokine family, with the highest homology with IL20.<br />This cytokine is released by activated monocytes, macrophages, and helper T cells (Th2) and is used in non-hematopoietic tissues such as skin, lung, and reproductive tissues. IL-24 plays an important role in wound healing, psoriasis and cancer treatment.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
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