Analysis of IL-28B/IFN-λ3 (Human) by ELISA (CAT#: STEM-MB-0975-LGZ)

Introduction

Interferon (IFN) receptor proteins are a class of cytokines secreted by host cells that regulate immune responses. The first cytokine to be discovered, it was named "interferon" because of the protein's ability to interfere with viral replication. When a pathogen is present, interferon is usually released by the host cell, which is sensed by surrounding uninfected cells and activates the appropriate cellular defense mechanisms in order to eliminate the pathogen.
IL-28B is also called IFNλ3. Human IL-28B gene encodes 200 amino acids including a 25 amino acid signal peptide and a mature protein with 175 amino acids lacking N-glycosylation sites.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Liver Injury, Oncology & Cancer, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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