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Analysis of INS Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2264-LGZ)

Introduction

Official Full Name: insulin<br />Also known as: IDDM; ILPR; IRDN; IDDM1; IDDM2; PNDM4; MODY10<br />This gene encodes insulin, a peptide hormone that plays a crucial role in regulating carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B-chain and A-chain peptides, which are covalently linked by two disulfide bonds to form insulin, and the C-peptide. Insulin binds to the insulin receptor (INSR) to stimulate glucose uptake. Numerous mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes, permanent neonatal diabetes, young type 10 diabetes, and hyperinsulinemia. There is one readable gene, INS-IGF2, which overlaps this gene in the 5' region and the IGF2 gene in the 3' region.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements