Analysis of ITGA10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1090-LGZ)

Introduction

Official Full Name: integrin subunit alpha 10<br />Also known as: PRO827<br />Integrins are complete transmembrane glycoproteins composed of non-covalently linked alpha and beta chains. They are involved in cell adhesion as well as cell surface mediated signaling. This gene encodes the integrin α chain, is expressed at a high level in chondrocytes, and is transcriptionally regulated by AP-2epsilon and Ets-1 in chondrocytes. The protein encoded by this gene binds to collagen. Alternative splicing results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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