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Analysis of KIF14 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1114-LGZ)

Introduction

Official Full Name: kinesin family member 14<br />Also known as: MKS12; MCPH20<br />This gene encodes a member of the microtubule motor protein kinase 3 superfamily. These proteins are involved in many processes including vesicle trafficking, chromosome segregation, mitotic spindle formation and cytoplasmic division. In human HeLa-S3 and 293T cells, the protein is localized to the cytoplasm during interphase, to the spindle and spindle microtubules during mitosis, and to the midbody during cytoplasmic division. The internal motor domain displays microtubule-dependent ATPase activity consistent with its function as a microtubule motor protein Knockdown of this gene results in failure of cell division, resulting in endo-replication, resulting in multinucleated cells. The gene has been identified as a possible oncogene in breast, lung, and ovarian cancers, as well as retinoblastoma and glioma. Alternative splicing results in multiple transcript variants.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements