Analysis of Leptospira Spp. by Real-Time PCR Method (CAT#: STEM-MB-3063-LGZ)

Introduction

Leptospira spp. are morphologically and phylogenetically unique bacteria that are ubiquitous in the environment. Pathogenic species are the agents of leptospirosis, an emerging zoonotic disease transmitted to both human and animals from the environment contaminated with the urine of reservoir animals.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.