Analysis of LGR6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0429-LGZ)

Introduction

Official Full Name: leucine rich repeat containing G protein-coupled receptor 6
Also known as: GPCR; VTS20631
This gene encodes a member of the leucine-rich repeat subgroup of the G protein-coupled 7 transmembrane protein superfamily. The encoded protein is a glycoprotein hormone receptor with a large N-terminal extracellular domain containing leucine-rich repeats important for forming a horseshoe-shaped ligand-binding interaction motif. Alternative splicing of this gene results in multiple transcript variants.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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