Analysis of LIMS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1465-LGZ)

Introduction

Official Full Name: LIM zinc finger domain containing 1
Also known as: PINCH; PINCH1; PINCH-1
The protein encoded by this gene is an adapter protein containing 5 LIM domains or double zinc fingers. This protein may be involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinases found in focal adhesion plaques. It is also considered to be a bridge between integrin-linked kinase and NCK adapter protein 2, which is involved in the growth factor receptor kinase signaling pathway. Its localization around spreading cells also suggests that the protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants of this gene have been found encoding different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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