Analysis of LY96 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1898-LGZ)

Introduction

Official Full Name: lymphocyte antigen 96
Also known as: MD2; MD-2; ly-96; ESOP-1
This gene encodes a protein which associates with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. Studies of the mouse ortholog suggest that this gene may be involved in endotoxin neutralization. Alternative splicing results in multiple transcript variants encoding different isoforms.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PTEN Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2056-LGZ)
Analysis of GSTM4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1194-LGZ)
Analysis of TRG-CCC1-1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1439-LGZ)
Analysis of AJAP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1238-LGZ)
Analysis of SCGB1C1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2123-LGZ)
Analysis of Giardia Lamblia TypeB by Real-Time PCR Method (CAT#: STEM-MB-3531-LGZ)
Analysis of Vibrio Alginolyticus by Real-Time PCR Method (CAT#: STEM-MB-3061-LGZ)
Analysis of SMG7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0311-LGZ)