Analysis of MAP3K21 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0404-LGZ)

Introduction

Official Full Name: mitogen-activated protein kinase kinase kinase 21<br />Also known as: MLK4; dJ862P8.3<br />Predicted to enable protein homodimerization activity and protein kinase activity. Predicted to be involved in protein autophosphorylation and signal transduction. Predicted to be active in cytoplasm.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Parechovirus (PeV) by Real-Time PCR Method (CAT#: STEM-MB-3418-LGZ)
Detection of Epidermal Growth Factor Receptor Gene Mutations in Lung Adenocarcinomas by Rapid Polymerase Chain Reaction (CAT#: STEM-MT-0010-LGZ)
Analysis of TRQ-CTG14-1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1438-LGZ)
Analysis of Taenia Spp. by Real-Time PCR Method (CAT#: STEM-MB-4029-LGZ)
Analysis of PAX6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2461-LGZ)
Analysis of CA14 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0817-LGZ)
Analysis of LOC126806577 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1621-LGZ)
Analysis of PDC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0558-LGZ)