Analysis of Midkine / MDK / NEGF2 by ELISA (CAT#: STEM-MB-1569-LGZ)

Introduction

Midkine (MK or MDK) is a 15 kDa heparin-binding molecule, also known as axon growth Promotion Factor 2 (NEGF2), which is a protein encoded by the MDK gene. Midkine is pleiotropic and can play a role in cell proliferation, cell migration, angiogenesis and fibrinolysis. Midkine expression has been found in various cancer types, such as neuroblastoma, colorectal cancer, liver cancer and ovarian cancer. The serum concentration of Midkine in patients with these malignant tumors is much higher than that of normal people. In some cases, these high concentrations of Midkine also predict a poorer prognosis for diseases such as neuroblastoma, glioma, and bladder cancer. Midkine acts as a low-affinity receptor that binds to nucleolins on the cell surface. This combination can inhibit HIV infection.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

Other recommended products

Analysis of Human CTSL(Cathepsin L) by ELISA (CAT#: STEM-MB-1718-LGZ)
Analysis of Rat GROγ/CXCL3(Growth Regulated Oncogene Gamma) by ELISA (CAT#: STEM-MB-2069-LGZ)
Analysis of Mouse TARC(Thymus Activation Regulated Chemokine) by ELISA (CAT#: STEM-MB-1913-LGZ)
Analysis of Aβ40 (4G8) (Human, Mouse, Rat) by ELISA (CAT#: STEM-MB-0680-LGZ)
Analysis of Plant β-(1,3)-D-glucans (1,3-β-DG) by ELISA (CAT#: STEM-MB-2423-LGZ)
Analysis of Human POSTN/OSF-2(Periostin) by ELISA (CAT#: STEM-MB-2195-LGZ)
Analysis of Plant α-amylase by ELISA (CAT#: STEM-MB-2340-LGZ)
Analysis of Rat LDHA(Lactate Dehydrogenase A) by ELISA (CAT#: STEM-MB-2134-LGZ)