Analysis of SDF-1α (Mouse) by ELISA (CAT#: STEM-MB-1176-LGZ)

Introduction

SDF-1α (stromal cell-derived factor 1α) is a member of the α subfamily of chemokines that lack an ELR domain. SDF-1α acts as a chemoattractant for T and B lymphocytes and monocytes. SDF-1 is an important member of the chemokine family, expressed in two subtypes, SDF-1α and SDF-1β, of which SDF-1α is the main subtype. SDF-1α is widely present in various tissues and organs of the human body, such as lymph nodes, bone marrow, liver, lung, muscle, small intestine, kidney, brain, etc., and can persist in these organs and tissues.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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