Analysis of MYCN Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1124-LGZ)

Introduction

Official Full Name: MYCN proto-oncogene, bHLH transcription factor
Also known as: NMYC; ODED; MODED; N-myc; bHLHe37; MYCNsORF; MYCNsPEP
This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Medulloblastoma, Glioblastoma, Pancreatic adenocarcinoma, Malignant neoplasm of body of uterus, Neuroblastoma

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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