Analysis of NCAM-1 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0451-LGZ)

Introduction

Nerve cell adhesion molecule 1(CD56) is mainly distributed in most cells, tissues and tumors of neuroectodermal origin, mainly used in retinoblastoma, medulloblastoma, astrocytoma, neuroblastoma and other tumors. The ligand is NCAM-1, and CD56 plays an important role in intercellular adhesion. The CD56 domain is homologous to the immunoglobulin domain.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Neurobiology/Neurodegeneration

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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