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Analysis of Serpin A12/Vaspin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0543-LGZ)

Introduction

Visceral fat-specific serine protease inhibitor (Vaspin, also known as Serpin A12), a 47kDa protein with 415 amino acids, belongs to the serine protease inhibitor (serpin) family and is also an interesting new adipocyte cytokine. The expression of Serpin A12 in abdominal adipose tissue is adipocyte-specific, and with the increase of visceral adipose tissue mass, the expression of Serpin A12 in subcutaneous adipose tissue will also decrease.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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