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Analysis of NCF2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0407-LGZ)

Introduction

Official Full Name: neutrophil cytosolic factor 2<br />Also known as: NCF-2; NOXA2; P67PHOX; P67-PHOX<br />This gene encodes neutrophil cytoplasmic factor 2, the 67 kilodalton cytoplasmic subunit of the multiprotein NADPH oxidase complex found in neutrophils. This oxidase generates large amounts of superoxide, which is transported into the lumen of the neutrophil phagosome. Mutations in this gene, as well as mutations in other NADPH oxidase subunits, cause chronic granulomatous disease, which causes recurrent infections with catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements