Analysis of Neurofilament L (Human) by ELISA (CAT#: STEM-MB-1094-LGZ)

Introduction

Neurofilament light chain (NFL or NEFL) is an important subunit of neuronal filament proteins (NF). As the main cytoskeleton protein of the axon of neurons, NF is highly expressed in the axon of neurons, and plays a role in maintaining the morphological stability of axons and ensuring the signal transmission of neurons. Under normal physiological conditions, the axon releases a small amount of NFL, but under pathological conditions, the amount of NFL is significantly increased. NFL in tissue fluid can spread to blood through cerebrospinal fluid, and the concentration of NFL in blood is lower than that in cerebrospinal fluid. The concentration of NFL in cerebrospinal fluid and blood is closely related to the degree of neuropathy, so NFL is used clinically as a biomarker of neuronal injury-related diseases.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Neurobiology/Neurodegeneration

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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