Analysis of NIBAN1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0487-LGZ)

Name: Analysis of NIBAN1 Gene (Mutation) by RT-qPCR
SKU: STEM-MT-0487-LGZ
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Introduction

Official Full Name: niban apoptosis regulator 1
Also known as: GIG39; NIBAN; C1orf24; FAM129A
This gene encodes a member of the family with sequence similarity 129 protein family. This gene is highly expressed in several cancer cells and may serve as a prognostic marker for certain cancers. The encoded protein may play a role in regulating p53-mediated apoptosis.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.