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Analysis of Notch-1/TAN1 by ELISA (CAT#: STEM-MB-1536-LGZ)

Introduction

Notch signaling pathway is a ubiquitous pathway that controls developmental processes. The Notch family consists of a group of highly conserved proteins that are both cell surface receptors and direct regulators of gene transcription. It plays an important regulatory role in cell proliferation, apoptosis, differentiation and a series of physiological and pathological processes, and plays a crucial role in the occurrence and development of cancer. Notch-1 is a 300kDa transmembrane type 1 glycoprotein, one of the four Notch homologues involved in developmental processes. Members of this type 1 transmembrane protein family share the same structural characteristics, including an extracellular domain composed of multiple epidermal growth factor-like (EGF) repeats and an intracellular domain composed of multiple different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. Notch-1 pathway is associated with cell differentiation, proliferation, apoptosis and adhesion. Abnormal activation of Notch-1 signaling pathway has been shown to be associated with the occurrence and development of cancer.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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