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Analysis of OGG1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1826-LGZ)

Introduction

Official Full Name: 8-oxoguanine DNA glycosylase<br />Also known as: HMMH; MUTM; OGH1; HOGG1<br />This gene encodes the enzyme responsible for cleaving 8-oxoguanine, a mutagenic byproduct of exposure to reactive oxygen species. The action of this enzyme includes the activity of lyase to cleave strands. Alternative splicing of the C-terminal region of this gene divides splice variants into two major categories, type 1 and type 2, based on the last exon of the sequence. Type 1 alternative splice variants end in exon 7 and type 2 end in exon 8. All variants share a common N-terminal region that contains the mitochondrial targeting signal, which is critical for mitochondrial localization. Many alternative splice variants of this gene have been described, but the full-length nature of each variant has not been determined.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements