Analysis of organic pollutant-protein interactions by UV-Vis Spectroscopy (CAT#: STEM-MB-0988-WXH)

Introduction

With the development of industry and human society, more attention was paid for the toxic effects of organic pollutants that are closely related to human daily life. Previous studies mainly focused on the dose-response relationship and cytotoxic effects of pollutants to organisms，while little research focused on pollutant-protein interactions at molecular level. However, the binding of organic pollutants to biomolecules, especially proteins like transporters, membrane receptor and nuclear receptors, is often the first step of toxic effects. It can make a series of endocrine disrupting and genotoxic effects through cell signaling pathway by binding specific target proteins including serum albumin, thyroid transporter, estrogen receptor, androgen receptor, and aryl hydrocarbon receptor. Thus, the research of interactions between organic pollutants and proteins is helpful and necessary to understand the distribution, metabolism and toxicity mechanism of compounds in organisms at the molecular level.