Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of P-Cadherin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0480-LGZ)

Introduction

Placental(P)-Cadherin(PCAD) belongs to the family of adhesion molecules and is a calcium-dependent transmembrane protein. Couples with catenins, α, β, and γ (plakoglobin) in the cytoplasm, and then associates with the cytoskeleton. Plays an important role in embryonic formation, especially tissue formation. It is also involved in maintaining the structure of mature tissues. Human PCAD has 829 amino acids in synthesis and 722 amino acids in mature protein.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
Advertisement