Analysis of PADI2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1347-LGZ)

Introduction

Official Full Name: peptidyl arginine deiminase 2
Also known as: PAD2; PDI2; PAD-H19
This gene encodes a member of the peptidylarginine deiminase family that catalyzes the post-translational deimination of proteins by converting arginine residues to citrulline in the presence of calcium ions. Family members have distinct substrate specificity and tissue-specific expression patterns. Type II enzymes are the most widely expressed family members. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the initiation and progression of neurodegenerative human diseases, including Alzheimer's disease and multiple sclerosis, and it has also been implicated in the pathogenesis of glaucoma. This gene exists in a cluster with four other homologous genes.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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